High-throughput cellular RNA device engineering
نویسندگان
چکیده
منابع مشابه
High-Throughput, Data-Rich Cellular RNA Device Engineering
Methods for rapidly assessing sequence-structure-function landscapes and developing conditional gene-regulatory devices are critical to our ability to manipulate and interface with biology. We describe a framework for engineering RNA devices from preexisting aptamers that exhibit ligandresponsive ribozyme tertiary interactions. Our methodology utilizes cell sorting, high-throughput sequencing, ...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2015
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.3486